DMWD Polyclonal antibody

DMWD Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16070-1-AP

Synonyms

D19S593E, DM9, DMR N9, DMRN9, DMWD, gene59, Protein 59, Protein DMR N9

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Validation Data Gallery

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  WB analysis using 16070-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16070-1-AP (DMWD antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 16070-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16070-1-AP (DMWD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 16070-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16070-1-AP (DMWD antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 16070-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16070-1-AP (DMWD antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 16070-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16070-1-AP (DMWD antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16070-1-AP targets DMWD in WB, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

• Tested Reactivity: human, rat, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DMWD fusion protein Ag9007
• Full Name: dystrophia myotonica, WD repeat containing
• Calculated Molecular Weight: 674 aa, 70 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC019266
• Gene Symbol: DMWD
• Gene ID (NCBI): 1762
• RRID: AB_2230476
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.