DMKN Polyclonal antibody

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  WB analysis of mouse ovary using 16252-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 16252-1-AP (DMKN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 16252-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16252-1-AP (DMKN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 16252-1-AP

  IP result of anti-DMKN (IP:16252-1-AP, 4ug; Detection:16252-1-AP 1:500) with mouse lung tissue lysate 4000ug.

DMKN Polyclonal Antibody for WB, IP, ELISA

Cat No. 16252-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Synonyms

Dermokine, Epidermis-specific secreted protein SK30/SK89, UNQ729/PRO1411

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Tested Applications

• Positive WB detected in: mouse lung tissue, mouse ovary tissue
• Positive IP detected in: mouse lung tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

16252-1-AP targets DMKN in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DMKN fusion protein Ag9242
• Full Name: dermokine
• Calculated Molecular Weight: 476 aa, 47 kDa
• Observed Molecular Weight: 48-55 kDa
• GenBank Accession Number: BC011886
• Gene Symbol: DMKN
• Gene ID (NCBI): 93099
• RRID: AB_10596473
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6E0U4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DMKN, also named as epidermis-specific secreted protein SK30/SK89, acts as a soluble regulator of keratinocyte differentiation. DMKN has 16 isoforms with MW 9-20 kDa, 35-38 kDa and 42-48 kDa.

Publications

Species	Application	Title
mouse	WB	Mol Cell Proteomics; Deletion of cysteine cathepsins B or L yields differential impacts on murine skin proteome and degradome.; Authors - Tholen Stefan S
		Mol Cell Proteomics; Matrix metalloproteinase 10 degradomics in keratinocytes and epidermal tissue identifies bioactive substrates with pleiotropic functions.; Authors - Pascal Schlage