DMGDH Polyclonal antibody, PBS Only

DMGDH Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

Cat no : 24813-1-PBS

Synonyms

ME2GLYDH, M2GD, EC:1.5.8.4, DMGDHD, dimethylglycine dehydrogenase

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Validation Data Gallery

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  WB analysis using 24813-1-AP (same clone as 24813-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24813-1-AP (DMGDH antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24813-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 24813-1-AP (same clone as 24813-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 24813-1-AP (DMGDH Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24813-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 24813-1-AP (same clone as 24813-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 24813-1-AP (DMGDH Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24813-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 24813-1-AP (same clone as 24813-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24813-1-AP (DMGDH Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 24813-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 24813-1-AP (same clone as 24813-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24813-1-AP (DMGDH Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 24813-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 24813-1-AP (same clone as 24813-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using DMGDH antibody (24813-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24813-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 24813-1-AP (same clone as 24813-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug DMGDH Polyclonal antibody (24813-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 24813-1-PBS in a different storage buffer formulation.

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Product Information

24813-1-PBS targets DMGDH in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DMGDH fusion protein Ag20529
• Full Name: dimethylglycine dehydrogenase
• Calculated Molecular Weight: 866 aa, 97 kDa
• Observed Molecular Weight: 97 kDa
• GenBank Accession Number: BC022388
• Gene Symbol: DMGDH
• Gene ID (NCBI): 29958
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

DMGDH(Dimethylglycine dehydrogenase, mitochondrial) is also named as ME2GLYDH and belongs to the GcvT family. It catalyzes the oxidative demethylation of dimethylglycine to form sarcosine and it plays a role in choline catabolism in the liver. The full length protein has a transit peptide.