DMGDH Polyclonal antibody
DMGDH Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, FC (Intra), ELISA
Conjugate
Unconjugated
Cat no : 24813-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse kidney tissue, mouse liver tissue, rat liver tissue, rat kidney tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
24813-1-AP targets DMGDH in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DMGDH fusion protein Ag20529 |
| Full Name | dimethylglycine dehydrogenase |
| Calculated Molecular Weight | 866 aa, 97 kDa |
| Observed Molecular Weight | 97 kDa |
| GenBank Accession Number | BC022388 |
| Gene Symbol | DMGDH |
| Gene ID (NCBI) | 29958 |
| RRID | AB_2879739 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DMGDH(Dimethylglycine dehydrogenase, mitochondrial) is also named as ME2GLYDH and belongs to the GcvT family. It catalyzes the oxidative demethylation of dimethylglycine to form sarcosine and it plays a role in choline catabolism in the liver. The full length protein has a transit peptide.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DMGDH antibody 24813-1-AP | Download protocol |
| IHC protocol for DMGDH antibody 24813-1-AP | Download protocol |
| IF protocol for DMGDH antibody 24813-1-AP | Download protocol |
| FC protocol for DMGDH antibody 24813-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice. | ||
Oncotarget Potential diagnostic and prognostic marker dimethylglycine dehydrogenase (DMGDH) suppresses hepatocellular carcinoma metastasis in vitro and in vivo. | ||
J Proteome Res Significant Down-Regulation of Urea Cycle Generates Clinically Relevant Proteomic Signature in Hepatocellular Carcinoma Patients with Macrovascular Invasion. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Hua (Verified Customer) (08-15-2024) | Great antibody. Strong signal and clean background
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