DMAP1 Monoclonal antibody

DMAP1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1A4E8

Cat no : 67183-1-Ig

Synonyms

DKFZp686L09142, DMAP1, DNMAP1, DNMT1 associated protein 1, DNMTAP1, EAF2, FLJ11543, KIAA1425, SWC4

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Validation Data Gallery

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  WB analysis using 67183-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67183-1-Ig (DMAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 67183-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using DMAP1 antibody (67183-1-Ig, Clone: 1A4E8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67183-1-Ig targets DMAP1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: DMAP1 fusion protein Ag28906
• Full Name: DNA methyltransferase 1 associated protein 1
• Calculated Molecular Weight: 53 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC002855
• Gene Symbol: DMAP1
• Gene ID (NCBI): 55929
• RRID: AB_2882479
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DMAP1 (DNA methyltransferase 1-associated protein 1) a component of the NuA4 histone acetyltransferase complex, was originally identified as an interacting molecule with DNA methyltransferase 1 (DNMT1) which co-localizes with PCNA at DNA replication foci. DMAP1 is required for DNA repair and acts against genomic instability. DMAP1 is widely implicated in transcription repression and activation via its interaction with multiple targets including histone deacetylase2 (HDAC2).