DLX5 Monoclonal antibody, PBS Only

DLX5 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C9G1

Cat no : 67111-1-PBS

Synonyms

distal less homeobox 5, DLX5, Homeobox protein DLX 5

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Validation Data Gallery

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  WB analysis using 67111-1-Ig (same clone as 67111-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67111-1-Ig (DLX5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67111-1-PBS in a different storage buffer formulation.

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  WB analysis of A375 using 67111-1-Ig (same clone as 67111-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 67111-1-Ig (DLX5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67111-1-PBS in a different storage buffer formulation.

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  WB analysis of U-251 using 67111-1-Ig (same clone as 67111-1-PBS)

  U-251 cells were subjected to SDS PAGE followed by western blot with 67111-1-Ig (DLX5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67111-1-PBS in a different storage buffer formulation.

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  WB analysis of THP-1 using 67111-1-Ig (same clone as 67111-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 67111-1-Ig (DLX5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67111-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67111-1-Ig (same clone as 67111-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67111-1-Ig (DLX5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67111-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67111-1-Ig (same clone as 67111-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67111-1-Ig (DLX5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67111-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67111-1-Ig (same clone as 67111-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67111-1-Ig (DLX5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67111-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67111-1-Ig (same clone as 67111-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67111-1-Ig (DLX5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67111-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67111-1-PBS targets DLX5 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: DLX5 fusion protein Ag17040
• Full Name: distal-less homeobox 5
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 33-38 kDa
• GenBank Accession Number: BC006226
• Gene Symbol: DLX5
• Gene ID (NCBI): 1749
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

DLX5 belongs to the DLX family of homeobox transcription factors (Dlx1, Dlx2, Dlx5 and Dlx6), which is expressed in overlapping domains at different stages of cell differentiation in the subpallium and controls differentiation of GABAergic neurons. DLX5 is an immediate early BMP-responsive transcriptional activator that essential for osteoblast differentiation. It can bind to and stimulate the homeodomain-response element of the ALPL and SP7 promoter. DLX5 can form homodimer with DLX2(~34kd) and heterodimer with MSX2(~29kd).