DLX5 Polyclonal antibody

• Featured Product
• KD/KO Validated

DLX5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 10592-1-AP

Synonyms

distal less homeobox 5, DLX5, Homeobox protein DLX 5

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Validation Data Gallery

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  WB analysis of A549 using 10592-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10592-1-AP (DLX5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 10592-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 10592-1-AP (DLX5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of U-251 using 10592-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 10592-1-AP (DLX5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human thymus using 10592-1-AP

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 10592-1-AP (DLX5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thymus using 10592-1-AP

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 10592-1-AP (DLX5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, A375 cells, U-251 cells
• Positive IHC detected in: human thymus tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below

Product Information

10592-1-AP targets DLX5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DLX5 fusion protein Ag0910
• Full Name: distal-less homeobox 5
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC006226
• Gene Symbol: DLX5
• Gene ID (NCBI): 1749
• RRID: AB_2090724
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DLX5 belongs to the DLX family of homeobox transcription factors (Dlx1, Dlx2, Dlx5 and Dlx6), which is expressed in overlapping domains at different stages of cell differentiation in the subpallium and controls differentiation of GABAergic neurons. DLX5 is an immediate early BMP-responsive transcriptional activator that essential for osteoblast differentiation. It can bind to and stimulate the homeodomain-response element of the ALPL and SP7 promoter. DLX5 can form homodimer with DLX2(~34kd) and heterodimer with MSX2(~29kd).

Publications

Species	Application	Title
human	IHC,WB	Nucleic Acids Res; Activation of bivalent factor DLX5 cooperates with master regulator TP63 to promote squamous cell carcinoma.; Authors - Yongsheng Huang ; KD Validated
rat	WB	Acta Biomater; Osteoinductivity and biomechanical assessment of a 3D printed demineralized bone matrix-ceramic composite in a rat spine fusion model.; Authors - Mark A Plantz
mouse	WB	J Cell Physiol; Long noncoding RNA FBXL19-AS1 induces tumor growth and metastasis by sponging miR-203a-3p in lung adenocarcinoma.; Authors - Liming Wang
human	IHC	Arthritis Res Ther; Ossification process involving the human thoracic ligamentum flavum: role of transcription factors.; Authors - Uchida Kenzo K
mouse	WB	PLoS One; Involvement of microRNAs in regulation of osteoblastic differentiation in mouse induced pluripotent stem cells.; Authors - Okamoto Hideharu H