DLK2 Polyclonal antibody

DLK2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 15680-1-AP

Synonyms

DLK 2, DLK2, EGF like protein 9, EGFL9, Protein delta homolog 2

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Validation Data Gallery

• 

  WB analysis of mouse brain using 15680-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15680-1-AP (DLK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 15680-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15680-1-AP (DLK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 15680-1-AP

  IP result of anti-DLK2 (IP:15680-1-AP, 4ug; Detection:15680-1-AP 1:500) with mouse brain tissue lysate 5600 ug.

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Tested Applications

• Positive WB detected in: mouse brain tissue, human liver tissue
• Positive IP detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

15680-1-AP targets DLK2 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DLK2 fusion protein Ag8170
• Full Name: delta-like 2 homolog (Drosophila)
• Calculated Molecular Weight: 383aa,41 kDa; 204aa,21 kDa
• Observed Molecular Weight: 41 kDa
• GenBank Accession Number: BC006425
• Gene Symbol: DLK2
• Gene ID (NCBI): 65989
• RRID: AB_2092804
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Int J Mol Sci; Different Expression Levels of DLK2 Inhibit NOTCH Signaling and Inversely Modulate MDA-MB-231 Breast Cancer Tumor Growth In Vivo.; Authors - Ana-Isabel Naranjo
mouse		Sci Rep; DLK proteins modulate NOTCH signaling to influence a brown or white 3T3-L1 adipocyte fate.; Authors - María-Luisa Nueda
rat	WB	Tissue Cell; Delta like Non-Canonical Notch Ligand 2 inhibits chondrogenic differentiation and cell proliferation of bone marrow mesenchymal stem cells through the Notch1 signaling pathway; Authors - Yanan Chen

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