SAP102 Monoclonal antibody

SAP102 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig, Mouse, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1F6A10

Cat no : 67102-1-Ig

Synonyms

Disks large homolog 3, DLG3, KIAA1232, MRX, MRX90, NE Dlg, NEDLG, Neuroendocrine DLG, SAP 102, SAP102, Synapse associated protein 102, XLMR

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Validation Data Gallery

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  WB analysis of pig brain using 67102-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67102-1-Ig (SAP102 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 67102-1-Ig

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 67102-1-Ig (SAP102 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 67102-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67102-1-Ig (SAP102 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 67102-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67102-1-Ig (SAP102 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67102-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67102-1-Ig (SAP102 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: pig brain tissue, mouse cerebellum tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67102-1-Ig targets SAP102 in WB, IHC, ELISA applications and shows reactivity with Human, Pig, Mouse, Rat samples.

• Tested Reactivity: Human, Pig, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SAP102 fusion protein Ag28589
• Full Name: discs, large homolog 3 (Drosophila)
• Calculated Molecular Weight: 817 aa, 90 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: BC093864
• Gene Symbol: SAP102
• Gene ID (NCBI): 1741
• RRID: AB_2882407
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Synapse-associated protein 102 (SAP102), also known as DLG3, is a MAGUK that is highly expressed early in development and mediates receptor trafficking during synaptogenesis. The importance of SAP102 function in brain development is demonstrated by the fact that SAP102 mutations have been identified in human patients with nonsyndromic X-linked mental retardation (XLMR).SAP102 has critical roles in early cortical synapse development.