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DLD Polyclonal antibody
DLD Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 16431-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | L02 cells, HeLa cells, human liver tissue, HepG2 cells, human brain tissue, mouse heart tissue, rat brain tissue, mouse liver tissue, rat heart tissue |
Positive IP detected in | mouse liver tissue |
Positive IHC detected in | human liver cancer tissue, human heart tissue, human kidney tissue, human testis tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 2 publications below |
WB | See 21 publications below |
IHC | See 3 publications below |
IP | See 1 publications below |
Product Information
16431-1-AP targets DLD in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | DLD fusion protein Ag9729 |
Full Name | dihydrolipoamide dehydrogenase |
Calculated Molecular Weight | 509 aa, 54 kDa |
Observed Molecular Weight | 56 kDa |
GenBank Accession Number | BC018696 |
Gene Symbol | DLD |
Gene ID (NCBI) | 1738 |
RRID | AB_2091888 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DLD(Dihydrolipoyl dehydrogenase, mitochondrial) is also named as GCSL, LAD, PHE3 and belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. It catalyzes the oxidation of dihydrolipoamide, hE3 uses two molecules : non-covalently bound FAD and a transiently bound substrate, NAD+. DLD is involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for DLD antibody 16431-1-AP | Download protocol |
IHC protocol for DLD antibody 16431-1-AP | Download protocol |
IF protocol for DLD antibody 16431-1-AP | Download protocol |
IP protocol for DLD antibody 16431-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Res Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation | ||
Nat Cell Biol RIP3 targets pyruvate dehydrogenase complex to increase aerobic respiration in TNF-induced necroptosis
| ||
J Hepatol OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism. | ||
Nat Commun Blocking glycine utilization inhibits multiple myeloma progression by disrupting glutathione balance.
| ||
Redox Biol Transketolase in human Müller cells is critical to resist light stress through the pentose phosphate and NRF2 pathways. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Hua (Verified Customer) (07-12-2023) | Great antibody for WB. Very strong signal and very clean background.
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