Validation Data Gallery
|Positive WB detected in||L02 cells, HeLa cells, human liver tissue, HepG2 cells, human brain tissue, mouse heart tissue, rat brain tissue, mouse liver tissue, rat heart tissue|
|Positive IP detected in||mouse liver tissue|
|Positive IHC detected in||human liver cancer tissue, human heart tissue, human kidney tissue, human testis tissue, human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
16431-1-AP targets DLD in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||DLD fusion protein Ag9729|
|Full Name||dihydrolipoamide dehydrogenase|
|Calculated molecular weight||509 aa, 54 kDa|
|Observed molecular weight||56 kDa|
|GenBank accession number||BC018696|
|Gene ID (NCBI)||1738|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
DLD(Dihydrolipoyl dehydrogenase, mitochondrial) is also named as GCSL, LAD, PHE3 and belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. It catalyzes the oxidation of dihydrolipoamide, hE3 uses two molecules : non-covalently bound FAD and a transiently bound substrate, NAD+. DLD is involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.
|Product Specific Protocols|
|WB protocol for DLD antibody 16431-1-AP||Download protocol|
|IHC protocol for DLD antibody 16431-1-AP||Download protocol|
|IF protocol for DLD antibody 16431-1-AP||Download protocol|
|IP protocol for DLD antibody 16431-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Hua (Verified Customer) (07-12-2023)
Great antibody for WB. Very strong signal and very clean background.