DKK4 Polyclonal antibody

DKK4 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 27080-1-AP

Synonyms

Dickkopf 4, Dickkopf related protein 4, DKK 4, DKK4, hDkk 4

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Validation Data Gallery

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  WB analysis of mouse cerebellum using 27080-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 27080-1-AP (DKK4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human oesophagus using 27080-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 27080-1-AP (DKK4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human oesophagus using 27080-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 27080-1-AP (DKK4 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse cerebellum tissue
• Positive IHC detected in: human oesophagus tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 27080-1-AP is DKK4 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DKK4 fusion protein Ag24209
• Full Name: dickkopf homolog 4 (Xenopus laevis)
• Calculated Molecular Weight: 224 aa, 25 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC107047
• Gene Symbol: DKK4
• Gene ID (NCBI): 27121
• RRID: AB_2880746
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB,IHC,IF	Clin Transl Med; O-GlcNAcylated LARP1 positively regulated by circCLNS1A facilitates hepatoblastoma progression through DKK4/β-catenin signalling; Authors - Zhongqi Cui