DKK3 Monoclonal antibody, PBS Only

DKK3 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4E6H6

Cat no : 66758-1-PBS

Synonyms

Dickkopf 3, Dickkopf related protein 3, Dkk 3, DKK3, hDkk 3, REIC, RIG

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Validation Data Gallery

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  WB analysis using 66758-1-Ig (same clone as 66758-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66758-1-Ig (DKK3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66758-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 66758-1-Ig (same clone as 66758-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66758-1-Ig (DKK3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66758-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 66758-1-Ig (same clone as 66758-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66758-1-Ig (DKK3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66758-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66758-1-Ig (same clone as 66758-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66758-1-Ig (DKK3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66758-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66758-1-Ig (same clone as 66758-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66758-1-Ig (DKK3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66758-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 66758-1-Ig (same clone as 66758-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using DKK3 antibody (66758-1-Ig, Clone: 4E6H6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66758-1-PBS in a different storage buffer formulation.

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Product Information

66758-1-PBS targets DKK3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: DKK3 fusion protein Ag23615
• Full Name: dickkopf homolog 3 (Xenopus laevis)
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 40 kDa, 50-55 kDa
• GenBank Accession Number: BC007660
• Gene Symbol: DKK3
• Gene ID (NCBI): 27122
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

DKK3, also named as REIC, belongs to the dickkopf family. It antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer's disea. The adenovirus carrying REIC/Dkk-3 (Ad-REIC) exhibits a potent tumor-specific cell-killing function for various human cancers. It has also become evident that some human cancers are resistant to Ad-REIC-induced apoptosis. The DKK3 is a Glycosylation protein with MW about 55 kDa or 38-43 kDa.