DKK3 Polyclonal antibody

• Featured Product
• KD/KO Validated

DKK3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

23

Cat no : 10365-1-AP

Synonyms

Dkk-3, Dkk 3, Dickkopf-related protein 3, Dickkopf-3, Dickkopf3

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Validation Data Gallery

• 

  WB analysis of MCF-7 using 10365-1-AP

  WB result of DKK3 antibody (10365-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DKK3 transfected MCF-7 cells.

• 

  WB analysis of HUVEC using 10365-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 10365-1-AP (DKK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 10365-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10365-1-AP (DKK3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10365-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10365-1-AP (DKK3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 10365-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 10365-1-AP (DKK3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HUVEC cells, MCF-7 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 9 publications below
• IF: See 7 publications below

Product Information

10365-1-AP targets DKK3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DKK3 fusion protein Ag0417
• Full Name: dickkopf homolog 3 (Xenopus laevis)
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 38-55 kDa
• GenBank Accession Number: BC007660
• Gene Symbol: DKK3
• Gene ID (NCBI): 27122
• RRID: AB_2261615
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DKK3, also named as REIC, belongs to the dickkopf family. It antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer's disea. The adenovirus carrying REIC/Dkk-3 (Ad-REIC) exhibits a potent tumor-specific cell-killing function for various human cancers. It has also become evident that some human cancers are resistant to Ad-REIC-induced apoptosis. The DKK3 is a Glycosylation protein with MW about 55 kDa or 38-43 kDa.

Publications

Species	Application	Title
human	IF	Nat Cell Biol; Single-cell reconstruction of the adult human heart during heart failure and recovery reveals the cellular landscape underlying cardiac function.; Authors - Li Wang
human,mouse	IHC	Sci Transl Med; Suppression of stromal-derived Dickkopf-3 (DKK3) inhibits tumor progression and prolongs survival in pancreatic ductal adenocarcinoma.; Authors - Liran Zhou ; KD Validated
		J Clin Invest; Phosphatase WIP1 regulates adult neurogenesis and WNT signaling during aging.; Authors - Yunhua Zhu
human	WB,IHC	Br J Cancer; microRNA-183 is an oncogene targeting Dkk-3 and SMAD4 in prostate cancer.; Authors - Ueno K K
rat	WB	J Cell Mol Med; Exercise ameliorates insulin resistance and improves ASK1-mediated insulin signalling in obese rats.; Authors - Yong Zhang
human	IHC	Mol Carcinog; Wnt antagonist DICKKOPF-3 (Dkk-3) induces apoptosis in human renal cell carcinoma.; Authors - Ueno Koji K

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