DIO2 Monoclonal antibody

• Featured Product
• KD/KO Validated

DIO2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse and More (1)

Applications

WB, IHC, RIP, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

1F2A1

Cat no : 66813-1-Ig

Synonyms

5DII, D2, DIO2, DIOII, ITDI2, SelY, TXDI2, Type 2 DI, Type II 5 deiodinase

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Validation Data Gallery

• 

  WB analysis of T-47D using 66813-1-Ig

  T-47D cells were subjected to SDS PAGE followed by western blot with 66813-1-Ig (DIO2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 66813-1-Ig

  WB result of DIO2 antibody (66813-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DIO2 transfected MCF-7 cells.

• 

  WB analysis of LNCaP using 66813-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 66813-1-Ig (DIO2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human thyroid cancer using 66813-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66813-1-Ig (DIO2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 66813-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66813-1-Ig (DIO2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 66813-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66813-1-Ig (DIO2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 66813-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66813-1-Ig (DIO2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: T-47D cells, LNCaP cells, MCF-7 cells
• Positive IHC detected in: human thyroid cancer tissue, mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• RIP: See 1 publications below

Product Information

66813-1-Ig targets DIO2 in WB, IHC, RIP, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: DIO2 fusion protein Ag24057
• Full Name: deiodinase, iodothyronine, type II
• Calculated Molecular Weight: 273 aa, 31 kDa
• Observed Molecular Weight: 30-35 kDa
• GenBank Accession Number: BC074882
• Gene Symbol: DIO2
• Gene ID (NCBI): 1734
• RRID: AB_2882156
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Type II iodothyronine deiodinase(DIO2), belongs to the iodothyronine deiodinase family, with two isoform of 30 and 34 kDa. DIO2 can activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 3,3',5-triiodothyronine (T3). DIO2 is thought to be responsible for the 'local' production of T3, and thus important in influencing thyroid hormone action in these tissues.

Publications

Species	Application	Title
human	WB	Autophagy; Defective autophagy contributes to endometrial epithelial-mesenchymal transition in intrauterine adhesions.; Authors - Zhenhua Zhou ; KD Validated
human	IHC	Biochem Biophys Rep; A prediction model for prognosis of gastric adenocarcinoma based on six metabolism-related genes; Authors - Jingyu Zhao
human	WB,RIP,IHC	Biochem Pharmacol; Dysregulated lncSNHG12 suppresses the invasion and migration of trophoblasts by regulating Dio2/Snail axis to involve in recurrent spontaneous abortion; Authors - Zehao Wang ; KD Validated
mouse	WB	Thyroid; Increased thyroid hormone action alleviates hippocampal damage by down-regulating neuronal type-Ⅰ interferon signaling/necroptosis in diabetes-associated cognitive dysfunction; Authors - Ling Tian