DHX38 Polyclonal antibody

• Featured Product
• KD/KO Validated

DHX38 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 10098-2-AP

Synonyms

DDX38, DEAH box protein 38, DHX38, KIAA0224, PRP16, PRPF16

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Validation Data Gallery

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  WB analysis using 10098-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10098-2-AP (DHX38 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10098-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10098-2-AP (DHX38 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10098-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10098-2-AP (DHX38 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10098-2-AP

  IP result of anti-DHX38 (IP:10098-2-AP, 4ug; Detection:10098-2-AP 1:700) with HeLa cells lysate 1400ug.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, HeLa cells, NIH/3T3 cells
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Product Information

10098-2-AP targets DHX38 in WB, IP, IF, IHC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DHX38 fusion protein Ag0144
• Full Name: DEAH (Asp-Glu-Ala-His) box polypeptide 38
• Calculated Molecular Weight: 141 kDa
• Observed Molecular Weight: 141 kDa
• GenBank Accession Number: BC004235
• Gene Symbol: DHX38
• Gene ID (NCBI): 9785
• RRID: AB_2092294
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The protein encoded by this gene is a member of the DEAD/H box family of splicing factors, which are involved in pre-mRNA splicing. The yeast prp16 is an essential splicing factor that catalyzes RNA-dependent hydrolysis of nucleoside triphosphates, acting as an ATPase. This protein resembles yeast Prp16 more closely than other DEAD/H family members and also is a general splicing factor essential for catalytic step II during pre-mRNA processing. Western blot analysis showed it is a 140-KD nuclear protein expressed in various tissues.

Publications

Species	Application	Title
human	WB	Gastroenterology; Solute Carrier Organic Anion Transporter Family Member 3A1 Is a Bile Acid Efflux Transporter in Cholestasis.; Authors - Qiong Pan
mouse	WB,IF,IP,ChIP	Nat Cell Biol; Blimp1 associates with Prmt5 and directs histone arginine methylation in mouse germ cells.; Authors - Ancelin Katia K
		Stem Cells; Characterisation of the Epigenetic Changes During Human Gonadal Primordial Germ Cells Reprogramming.; Authors - C Eguizabal
human	WB	iScience; The splicing factor DHX38 enables retinal development through safeguarding genome integrity; Authors - Kui Sun ; KD Validated
mouse	IF	PLoS One; Reprogramming primordial germ cells into pluripotent stem cells.; Authors - Durcova-Hills Gabriela G
human	WB	FEBS Open Bio; The splicing factor DHX38/PRP16 is required for ovarian clear cell carcinoma tumorigenesis, as revealed by a CRISPR-Cas9 screen.; Authors - Brandon Cona ; KD Validated