DHX37 Polyclonal antibody

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  WB analysis using 29741-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29741-1-AP (DHX37 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 29741-1-AP

  WB result of DHX37 antibody (29741-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DHX37 transfected HEK-293 cells.

• Featured Product
• KD/KO Validated

DHX37 Polyclonal Antibody for WB, ELISA

Cat No. 29741-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, ELISA

Synonyms

DDX37, DEAH box protein 37, DHX37, FLJ41974, KIAA1517

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

29741-1-AP targets DHX37 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DHX37 fusion protein Ag29909
• Full Name: DEAH (Asp-Glu-Ala-His) box polypeptide 37
• Calculated Molecular Weight: 1157 aa, 130 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC037964
• Gene Symbol: DHX37
• Gene ID (NCBI): 57647
• RRID: AB_2923602
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IY37
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DHX37, also known as DDX37, KIAA1517. It is an ATP-bound RNA helicase that plays a role in the maturation of small ribosomal subunits in ribosomal biogenesis (PMID:30582406). The presence of DHX37 in early pre-ribosomal particles was monitored by a quality control pathway, and failure to recruit DHX37 resulted in pre-RRNA degradation. DHX37 binds directly to U3 small nucleolar RNA (snoRNA), and the catalytic activity of the helicase is required for dissociation of U3 small nucleolar RNA from the pre-ribosomal complex.In addition, it plays a role in early testicular development (PMID:31287541, PMID:31337883). It may also have an effect on brain development (PubMed:31256877). It is expressed in the fallopian tubes, ovaries, uterus, testes and brain (PMID: 31256877,PMID: 31337883).

Publications

Species	Application	Title
human	WB	Cancers (Basel); DHX37 Is a Promising Prognostic Biomarker and a Therapeutic Target for Immunotherapy and Chemotherapy in HCC; Authors - Nanbin Liu