DHTKD1 Polyclonal antibody

DHTKD1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 27493-1-AP

Synonyms

DHTKD1, KIAA1630

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Validation Data Gallery

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  WB analysis using 27493-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27493-1-AP (DHTKD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27493-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27493-1-AP (DHTKD1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-13 using 27493-1-AP

  PC-13 cells were subjected to SDS PAGE followed by western blot with 27493-1-AP (DHTKD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 27493-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27493-1-AP (DHTKD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 27493-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27493-1-AP (DHTKD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 27493-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using DHTKD1 antibody (27493-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, PC-13 cells, L02 cells, mouse kidney tissue, mouse liver tissue, rat liver tissue
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

27493-1-AP targets DHTKD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DHTKD1 fusion protein Ag26515
• Full Name: dehydrogenase E1 and transketolase domain containing 1
• Calculated Molecular Weight: 103 kDa
• Observed Molecular Weight: 103 kDa
• GenBank Accession Number: BC007955
• Gene Symbol: DHTKD1
• Gene ID (NCBI): 55526
• RRID: AB_2880888
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Front Endocrinol (Lausanne); Knock-Out of DHTKD1 Alters Mitochondrial Respiration and Function, and May Represent a Novel Pathway in Cardiometabolic Disease Risk.; Authors - Chuan Wang
mouse	WB,IHC	J Hepatocell Carcinoma; Liver Regeneration-Related Genes of Nontumor Liver Tissues Predict the Prognosis of Patients with Hepatocellular Carcinoma; Authors - Yanchao Xu