DHRS3 Recombinant antibody, PBS Only (Detector)

DHRS3 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240545D8

Cat no : 83581-3-PBS

Synonyms

Retinol dehydrogenase 17, Retinal short-chain dehydrogenase/reductase 1, RDH17, EC:1.1.1.300, DD83.1

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Validation Data Gallery

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  WB analysis using 83581-3-RR (same clone as 83581-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83581-3-RR (DHRS3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83581-3-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83581-3-RR (same clone as 83581-3-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DHRS3 antibody (83581-3-RR, Clone: 240545D8 ) at dilution of 1:100 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 83581-3-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 83581-3-RR (same clone as 83581-3-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug DHRS3 Recombinant antibody (83581-3-RR, Clone:240545D8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83581-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00554-1

  Cytometric bead array standard curve of MP00554-1, DHRS3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83581-2-PBS. Detection antibody: 83581-3-PBS. Standard: Ag7589. Range: 0.313-40 ng/mL.

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  Cytometric bead array standard curve of MP00554-3

  Cytometric bead array standard curve of MP00554-3, DHRS3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83581-1-PBS. Detection antibody: 83581-3-PBS. Standard: Ag7589. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 83581-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83548-3-RR against Human GHITM were performed. The affinity constant is 2.41 nM. Biolayer interferometry (BLl) kinetic assays of 83581-3-RR against Human DHRS3 were performed. The affinity constant is 0.109 nM.

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Product Information

83581-3-PBS targets DHRS3 as part of a matched antibody pair:

MP00554-1: 83581-2-PBS capture and 83581-3-PBS detection (validated in Cytometric bead array)

MP00554-3: 83581-1-PBS capture and 83581-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: DHRS3 fusion protein Ag7589
• Full Name: dehydrogenase/reductase (SDR family) member 3
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 30-34 kDa
• GenBank Accession Number: BC002730
• Gene Symbol: DHRS3
• Gene ID (NCBI): 9249
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

DHRS3(Short-chain dehydrogenase/reductase 3) is also named as retSDR1,DD83.1 and belongs to the short-chain dehydrogenases/reductases (SDR) family. It catalyzes the reduction of all-trans-retinal to all-trans-retinol in the presence of NADPH. It is detected high levels of expression in fetal kidney, liver, and lung and in adult heart, placenta, lung, liver, kidney, pancreas, thyroid, testis, stomach, trachea, spinal cord and lower levels in skeletal muscle, intestine, and lymph node. retSDR1 is barely detectable in adrenals, brain, thymus, and hematopoietic tissues(PMID:11861404). retSDR1 may play a more general role in retinol metabolism since its expression was observed in many fetal and adult tissues(PMID:20543567).