DHRS3 Polyclonal antibody

• Featured Product
• KD/KO Validated

DHRS3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

19

Cat no : 15393-1-AP

Synonyms

retSDR1, Retinol dehydrogenase 17, Retinal short-chain dehydrogenase/reductase 1, RDH17, EC:1.1.1.300

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Validation Data Gallery

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  WB analysis using 15393-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15393-1-AP (DHRS3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 15393-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15393-1-AP (DHRS3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of A375 using 15393-1-AP

  IP result of anti-DHRS3 (IP:15393-1-AP, 4ug; Detection:15393-1-AP 1:500) with A375 cells lysate 3600ug.

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  IHC staining of mouse liver using 15393-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15393-1-AP (DHRS3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 15393-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15393-1-AP (DHRS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 15393-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DHRS3 antibody (15393-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A375 cells, mouse liver tissue, rat liver tissue, mouse kidney tissue
• Positive IP detected in: A375 cells
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 16 publications below
• IHC: See 2 publications below
• IF: See 5 publications below

Product Information

15393-1-AP targets DHRS3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, insect
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DHRS3 fusion protein Ag7589
• Full Name: dehydrogenase/reductase (SDR family) member 3
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 30-34 kDa
• GenBank Accession Number: BC002730
• Gene Symbol: DHRS3
• Gene ID (NCBI): 9249
• RRID: AB_2091855
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DHRS3(Short-chain dehydrogenase/reductase 3) is also named as retSDR1,DD83.1 and belongs to the short-chain dehydrogenases/reductases (SDR) family. It catalyzes the reduction of all-trans-retinal to all-trans-retinol in the presence of NADPH. It is detected high levels of expression in fetal kidney, liver, and lung and in adult heart, placenta, lung, liver, kidney, pancreas, thyroid, testis, stomach, trachea, spinal cord and lower levels in skeletal muscle, intestine, and lymph node. retSDR1 is barely detectable in adrenals, brain, thymus, and hematopoietic tissues(PMID:11861404). retSDR1 may play a more general role in retinol metabolism since its expression was observed in many fetal and adult tissues(PMID:20543567).

Publications

Species	Application	Title
human	WB	J Clin Endocrinol Metab; Elevated periimplantation uterine natural killer cell density in human endometrium is associated with impaired corticosteroid signaling in decidualizing stromal cells.; Authors - Kuroda Keiji K
mouse	IHC	FASEB J; The retinaldehyde reductase DHRS3 is essential for preventing the formation of excess retinoic acid during embryonic development.; Authors - Billings Sara E SE
human,mouse	WB	J Biol Chem; Retinol dehydrogenase 11 is essential for the maintenance of retinol homeostasis in liver and testis in mice.; Authors - Olga V Belyaeva ; KD Validated
mouse	WB	FASEB J; Liver regeneration and ethanol detoxification: A new link in YAP regulation of ALDH1A1 during alcohol-related hepatocyte damage.; Authors - Junmei Zhou
human	WB	Mol Endocrinol; Induction of 11β-HSD 1 and activation of distinct mineralocorticoid receptor- and glucocorticoid receptor-dependent gene networks in decidualizing human endometrial stromal cells.; Authors - Kuroda Keiji K
human	WB,IF	J Biol Chem; Retinol dehydrogenase 10 but not retinol/sterol dehydrogenase(s) regulates the expression of retinoic acid-responsive genes in human transgenic skin raft culture.; Authors - Lee Seung-Ah SA