DHODH Monoclonal antibody, PBS Only

DHODH Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B2C6

Cat no : 67977-1-PBS

Synonyms

DHOdehase, DHODH, dihydroorotate dehydrogenase, Dihydroorotate oxidase

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Validation Data Gallery

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  WB analysis of LNCaP using 67977-1-Ig (same clone as 67977-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67977-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HSC-T6 using 67977-1-Ig (same clone as 67977-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67977-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 67977-1-Ig (same clone as 67977-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67977-1-PBS in a different storage buffer formulation.

• 

  WB analysis of 4T1 using 67977-1-Ig (same clone as 67977-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67977-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67977-1-Ig (same clone as 67977-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67977-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 67977-1-Ig (same clone as 67977-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67977-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 67977-1-Ig (same clone as 67977-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 67977-1-Ig (DHODH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67977-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67977-1-PBS targets DHODH in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: DHODH fusion protein Ag6649
• Full Name: dihydroorotate dehydrogenase
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC065245
• Gene Symbol: DHODH
• Gene ID (NCBI): 1723
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

DHODH(Dihydroorotate dehydrogenase) catalyzes the fourth enzymatic step in de novo pyrimidine biosynthesis.DHO dehydrogenase is a monofunctional protein which, in most eukaryotic organisms, is located on the outer surface of the inner mitochondrial membrane.Defects in DHODH are the cause of postaxial acrofacial dysostosis (POADS).