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DHH Polyclonal antibody, PBS Only

DHH Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13889-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skeletal muscle tissue using DHH Polyclonal antibody (13889-1-AP)

    WB analysis of mouse skeletal muscle using 13889-1-AP (same clone as 13889-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13889-1-AP (DHH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13889-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using DHH Polyclonal antibody (13889-1-AP)

    WB analysis of mouse skeletal muscle using 13889-1-AP (same clone as 13889-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13889-1-AP (DHH antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13889-1-PBS in a different storage buffer formulation.

Product Information

13889-1-PBS targets DHH in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DHH fusion protein Ag4850
Full Name desert hedgehog homolog (Drosophila)
Calculated Molecular Weight 396 aa, 44 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC033507
Gene Symbol DHH
Gene ID (NCBI) 50846
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Desert hedgehog (DHH) is one of three ligands in the hedgehog signaling pathway that controls gonad 

morphogenesis and nerve perineurium differentiation.


What is the molecular weight of DHH? Is DHH post-translationally modified?

DHH protein is synthesized in a precursor form of ~45 kDa. Hedgehog proteins contain a small N-terminal signal 

peptide, which is cleaved off intracellularly, leading to 39 kDa species. Hedgehog proteins are then subject to 

autoproteolysis, which leads to the generation of a 19 kDa N-terminal fragment with added cholesterol moiety 

and a 25 kDa C-terminal fragment (PMID: 7985023). The C-terminal fragment catalyzes the precursor proteolysis, 

while the N-terminal fragment gets secreted from cells and acts as an active ligand in the hedgehog signaling 

pathway.


What is the subcellular localization of DHH?

DHH protein is subject to autoproteolysis, as described above, and the N-terminal fragment is secreted from 

cells in a regulated manner. The amounts of extracellular hedgehog proteins determine local and long-range 

signaling (PMID: 7736596 and 7885476).


What is the tissue expression pattern of DHH?

Unlike other hedgehog ligands, expression of DHH is very tissue-specific. DHH is strongly expressed in Steroli 

cell precursors, adult sterol cells, and in Leydig cells in testis (PMID: 8805249, 12050120) but also in granulosa 

cells in ovaries (PMID: 15878962), as well as in Schwann cells-glia of the peripheral nervous system 

(PMID: 7589793).


What is the role of DHH in morphogenesis?

DHH plays a role in the correct development of male gonads and perineurium. Loss of DHH leads to peripheral 

neuropathy and gonadal dysgenesis (PMID: 11017805).