DHDDS Polyclonal antibody

DHDDS Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 15099-1-AP

Synonyms

Epididymis tissue protein Li 189m, EC:2.5.1.87, Dehydrodolichyl diphosphate synthase complex subunit DHDDS, Cis-prenyltransferase subunit hCIT, Cis-isoprenyltransferase

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Validation Data Gallery

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  WB analysis using 15099-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15099-1-AP (DHDDS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, Y79 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15099-1-AP targets DHDDS in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DHDDS fusion protein Ag7426
• Full Name: dehydrodolichyl diphosphate synthase
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC003643
• Gene Symbol: DHDDS
• Gene ID (NCBI): 79947
• RRID: AB_2091576
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DHDDS (dehydrodolichol diphosphate synthetase) encodes a protein involved in dolichol biosynthesis on the cytoplasmic face of the ER, where N-glycosylation, O-mannosylation, C-mannosylation and GPI-anchor synthesis occurs (PMID: 34382076). Homozygous mutations in the DHDDS gene (c.124 A>G) have been associated with nonsyndromic retinitis pigmentosa in consanguineous families (PMID: 36628425).