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DHCR24 Polyclonal antibody

DHCR24 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10471-1-AP

Synonyms

DHCR24, Diminuto/dwarf1 homolog, KIAA0018, Nbla03646, Seladin 1, SELADIN1



Tested Applications

Positive WB detected inHepG2 cells, mouse lung tissue, Jurkat cells, MCF-7 cells
Positive IHC detected inhuman liver tissue, mouse liver tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10471-1-AP targets DHCR24 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DHCR24 fusion protein Ag0756
Full Name 24-dehydrocholesterol reductase
Calculated Molecular Weight 60 kDa, 516 aa
Observed Molecular Weight 50-60 kDa
GenBank Accession NumberBC004375
Gene Symbol DHCR24
Gene ID (NCBI) 1718
RRIDAB_2091446
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DHCR24(3-beta-hydroxysterol delta-24-reductase), also named as Seladin-1 or Diminuto/dwarf1 homolog, is a member of the flavin adenine dinucleotide (FAD)-dependent oxidoreductases, and catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The DHCR24 expression was shown to be down regulated in brain areas affected by Alzheimer's disease and endogenous DHCR24/seladin-1 levels are highly up-regulated upon acute oxidative stress while expression declines to very low levels upon chronic exposure (PMID:17984220). In apoptotic cells, endogenous seladin-1 was cleaved to a 40 kDa derivative in a caspase-dependent manner (PMID: 11007892).

Protocols

Product Specific Protocols
WB protocol for DHCR24 antibody 10471-1-APDownload protocol
IHC protocol for DHCR24 antibody 10471-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Nature

Feeding induces cholesterol biosynthesis via the mTORC1-USP20-HMGCR axis.

Authors - Xiao-Yi Lu
mouseWB

EMBO Mol Med

Shaping immune landscape of colorectal cancer by cholesterol metabolites

Authors - Yibing Bai
humanWB

Cell Death Differ

Efferocytosis potentiates the expression of arachidonate 15-lipoxygenase (ALOX15) in alternatively activated human macrophages through LXR activation.

Authors - Ryan G Snodgrass
humanWB

Commun Biol

Cholesterol mediated ferroptosis suppression reveals essential roles of Coenzyme Q and squalene

Authors - Qi Sun
  • KD Validated

J Neurosci

Golgi-Dependent Copper Homeostasis Sustains Synaptic Development and Mitochondrial Content.

Authors - Cortnie Hartwig
mouseWB

NanoImpact

The alterations of miRNA and mRNA expression profile and their integration analysis induced by silica nanoparticles in spermatocyte cells.

Authors - Guiqing Zhou