dGK Polyclonal antibody

dGK Polyclonal Antibody for IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16459-1-AP

Synonyms

DGUOK, EC:2.7.1.76, EC:2.7.1.113, Deoxyguanosine kinase, Deoxyadenosine kinase, mitochondrial

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Validation Data Gallery

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  IP experiment of mouse kidney using 16459-1-AP

  IP result of anti-dGK (IP:16459-1-AP, 3ug; Detection:16459-1-AP 1:300) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human skin cancer using 16459-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 16459-1-AP (dGK antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16459-1-AP targets dGK in IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: dGK fusion protein Ag9501
• Full Name: deoxyguanosine kinase
• Calculated Molecular Weight: 277 aa, 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC015757
• Gene Symbol: DGUOK
• Gene ID (NCBI): 1716
• RRID: AB_2878260
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.