DGKZ Polyclonal antibody

DGKZ Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 32124-1-AP

Synonyms

Diacylglycerol kinase zeta, DGK-zeta, DGK ZETA, DAGK6, DAGK5

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Validation Data Gallery

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  WB analysis using 32124-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32124-1-AP (DGKZ antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 32124-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using DGKZ antibody (32124-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32124-1-AP targets DGKZ in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DGKZ fusion protein Ag37336
• Full Name: diacylglycerol kinase, zeta 104kDa
• Calculated Molecular Weight: 104kDa，928aa
• Observed Molecular Weight: 104-124 kDa
• GenBank Accession Number: NM_001199267.1
• Gene Symbol: DGKZ
• Gene ID (NCBI): 8525
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Diacylglycerol kinase zeta (DGKZ), also known as DAGK5, DAGK6, DGK-ZETA, hDGKzeta, belongs to the diacylglycerol kinase (DGK) family which catalyzes diacylglycerol (DAG) into phosphatidic acid (PA). DGKZ is a novel promoter of metastasis and that it could be a potential prognostic indicator in patients with triple-negative breast cancer by suppressing lipid raft-dependent endocytosis of TGFbetaR2(PMID: 35115500). Downregulation of DGKZ suppresses tumorigenesis and progression of cervical cancer by facilitating cell apoptosis and cell cycle arrest(PMID: 33926342).