DGKQ Polyclonal antibody

• Featured Product
• KD/KO Validated

DGKQ Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 17885-1-AP

Synonyms

DGK-theta, DGKtheta, DGK theta, DAGK7, DAGK4

---

Validation Data Gallery

• 

  WB analysis of mouse brain using 17885-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17885-1-AP (DGKQ antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17885-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 17885-1-AP (DGKQ antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 17885-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 17885-1-AP (DGKQ antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 17885-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17885-1-AP (DGKQ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 17885-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17885-1-AP (DGKQ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17885-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17885-1-AP (DGKQ antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervix using 17885-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervix using 17885-1-AP (DGKQ antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human cervix using 17885-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervix using 17885-1-AP (DGKQ antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse brain using 17885-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17885-1-AP (DGKQ antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse small intestine using 17885-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using DGKQ antibody (17885-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse kidney using 17885-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using DGKQ antibody (17885-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: mouse brain tissue, human placenta tissue, THP-1 cells
• Positive IHC detected in: human colon cancer tissue, human cervix tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse small intestine tissue, mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

17885-1-AP targets DGKQ in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DGKQ fusion protein Ag11938
• Full Name: diacylglycerol kinase, theta 110kDa
• Calculated Molecular Weight: 942 aa, 101 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC063801
• Gene Symbol: DGKQ
• Gene ID (NCBI): 1609
• RRID: AB_2091305
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Mol Med Rep; Establishment of a novel hepatic steatosis cell model by Cas9/sgRNA-mediated DGKθ gene knockout.; Authors - Jingjing Zhang ; KO Validated
	WB	J Sci Food Agric; Neonatal resveratrol administration promotes skeletal muscle growth and insulin sensitivity in intrauterine growth-retarded suckling piglets associated with activation of FGF21-AMPKα pathway; Authors - Guangyi Bai