DGKH Polyclonal antibody

• Featured Product
• KD/KO Validated

DGKH Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 13873-1-AP

Synonyms

EC:2.7.1.107, DGK-eta, DGKeta, DAG kinase eta

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Validation Data Gallery

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  WB analysis of mouse brain using 13873-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13873-1-AP (DGKH antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 13873-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13873-1-AP (DGKH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13873-1-AP

  IP result of anti-DGKH (IP:13873-1-AP, 5ug; Detection:13873-1-AP 1:500) with mouse brain tissue lysate 2640ug.

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  IHC staining of human testis using 13873-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13873-1-AP (DGKH Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 13873-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13873-1-AP (DGKH Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of SH-SY5Y using 13873-1-AP

  Immunofluorescent analysis of (-20℃ Acetone) fixed SH-SY5Y cells using 13873-1-AP (DGKH antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

13873-1-AP targets DGKH in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DGKH fusion protein Ag4926
• Full Name: diacylglycerol kinase, eta
• Calculated Molecular Weight: 1100 aa, 122 kDa
• Observed Molecular Weight: 135 kDa
• GenBank Accession Number: BC043292
• Gene Symbol: DGKH
• Gene ID (NCBI): 160851
• RRID: AB_2091287
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DGKH(Diacylglycerol kinase eta) is also named as DGK-eta and belongs to the eukaryotic diacylglycerol kinase family. It is a key protein in the lithium-sensitive phosphatidyl inositol pathway, and recently a link between environmental stress and schizophrenia/bipolar disorder has been revealed. DGKH has some isoforms produced by alternative splicing. Two isoforms DGKH1 and DGKH2 with the molecular weight of 128 kDa and 135 kDa shows diacylglycerol kinase activity; however, the 2 isoforms differed in several other characteristics(PMID:12810723).

Publications

Species	Application	Title
human, mouse	WB	J Invest Dermatol; Diacylglycerol kinase regulates tyrosinase expression and function in human melanocytes.; Authors - Kawaguchi Masakazu M
mouse	WB	BMC Dev Biol; Distinct expression and localization of the type II diacylglycerol kinase isozymes ¿, ¿ and ¿ in the mouse reproductive organs.; Authors - Takao Shionoya
mouse	WB	J Histochem Cytochem; Expression and Localization of Type II Diacylglycerol Kinase Isozymes δ and η in the Developing Mouse Brain.; Authors - Takako Usuki
human, mouse	WB,IHC	Clin Transl Oncol; Diacylglycerol kinase η modulates oncogenic properties of lung cancer cells.; Authors - Nakano T T ; KD Validated
mouse	WB	Biochim Biophys Acta Mol Cell Biol Lipids; Myristic acid specifically stabilizes diacylglycerol kinase δ protein in C2C12 skeletal muscle cells.; Authors - Kai Iwata
human	WB	Cancer Med; Metabolism-related lncRNAs signature to predict the prognosis of colon adenocarcinoma; Authors - Yimin Sun