DGCR8 Monoclonal antibody

• Featured Product
• KD/KO Validated

DGCR8 Monoclonal Antibody for WB, IP, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IP, RIP, ELISA

Conjugate

Unconjugated

Publications

11

CloneNo.

4D11A3

Cat no : 60084-1-Ig

Synonyms

C22orf12, DGCR8, DGCRK6, Gy1

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Validation Data Gallery

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  WB analysis using 60084-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60084-1-Ig (DGCR8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 60084-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60084-1-Ig (DGCR8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 60084-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 60084-1-Ig (DGCR8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 60084-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60084-1-Ig (DGCR8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 60084-1-Ig

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 60084-1-Ig (DGCR8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 60084-1-Ig

  IP result of anti-DGCR8 (IP:60084-1-Ig, 5ug; Detection:60084-1-Ig 1:500) with K-562 cells lysate 3440ug.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, BxPC-3 cells, RAW 264.7 cells, A431 cells, Jurkat cells, K-562 cells, ROS1728 cells
• Positive IP detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IP: See 1 publications below
• RIP: See 2 publications below

Product Information

60084-1-Ig targets DGCR8 in WB, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: DGCR8 fusion protein Ag4871
• Full Name: DiGeorge syndrome critical region gene 8
• Calculated Molecular Weight: 773 aa, 86 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC009323
• Gene Symbol: DGCR8
• Gene ID (NCBI): 54487
• RRID: AB_2090985
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DGCR8 is a RNA-binding protein that assists the Rnase III enzyme Drosha in the processing of microRNAs (miRNAs), which regulate the expression of a large number of protein-coding genes[PMID: 22580560]. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, may be an essential component of the primary miRNAs processing complex, along with Drosha, promoting the processing of primary microRNA to precursor microRNA. It is ubiquitous expressed in human and mouse tissues, and is deleted in DiGeorge syndrome[22323604]. The calculated molecular weight of DGCR8 is 82-86 kDa, but the post-modified DGCR8 is about 120 kDa.

Publications

Species	Application	Title
mouse,human	WB	Nature; Global miRNA dosage control of embryonic germ layer specification.; Authors - Yingzi Cui ; KO Validated
mouse	WB	Mol Cell; A transient transcriptional activation governs unpolarized-to-polarized morphogenesis during embryo implantation; Authors - Xuehui Lyu
human	WB	Nat Commun; Hypoxia regulates overall mRNA homeostasis by inducing Met1-linked linear ubiquitination of AGO2 in cancer cells.; Authors - Hailong Zhang
human,rat	WB	Aging Cell; p38 MAPK-mediated loss of nuclear RNase III enzyme Drosha underlies amyloid beta-induced neuronal stress in Alzheimer's disease; Authors - Haidong Xu
human	WB,RIP	Mol Ther; DGCR8/ZFAT-AS1 Promotes CDX2 Transcription in a PRC2 Complex-Dependent Manner to Facilitate the Malignant Biological Behavior of Glioma Cells.; Authors - Fangfang Zhang
human, mouse	WB	Nucleic Acids Res; SUMOylation at K707 of DGCR8 controls direct function of primary microRNA.; Authors - Changhong Zhu