WB result of DGCR8 antibody (25835-1-AP, 1:2000) with si-control and si-DGCR8 transfected A431 cells.
WB analysis of HeLa using 25835-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 25835-1-AP (DGCR8 N-terminal antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 25835-1-AP (DGCR8 N-terminal antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 25835-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 25835-1-AP (DGCR8 N-terminal antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
Jurkat cells were subjected to SDS PAGE followed by western blot with 25835-1-AP (DGCR8 N-terminal antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 25835-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 25835-1-AP (DGCR8 N-terminal antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
HepG2 cells were subjected to SDS PAGE followed by western blot with 25835-1-AP (DGCR8 N-terminal antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 25835-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 25835-1-AP (DGCR8 N-terminal antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
A431 cells were subjected to SDS PAGE followed by western blot with 25835-1-AP (DGCR8 N-terminal antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 25835-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 25835-1-AP (DGCR8 N-terminal antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
HEK-293 cells were subjected to SDS PAGE followed by western blot with 25835-1-AP (DGCR8 N-terminal antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of A431 using 25835-1-AP
IP result of anti-DGCR8 N-terminal (IP:25835-1-AP, 4ug; Detection:25835-1-AP 1:600) with A431 cells lysate 2800ug.
IP result of anti-DGCR8 N-terminal (IP:25835-1-AP, 4ug; Detection:25835-1-AP 1:600) with A431 cells lysate 2800ug.
IHC staining of human ovary cancer using 25835-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25835-1-AP (DGCR8 N-terminal antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25835-1-AP (DGCR8 N-terminal antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 25835-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 25835-1-AP (DGCR8 N-terminal antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 25835-1-AP (DGCR8 N-terminal antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:1000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)/ICC
IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
DGCR8 is a RNA-binding protein that assists the Rnase III enzyme Drosha in the processing of microRNAs (miRNAs), which regulate the expression of a large number of protein-coding genes[PMID: 22580560]. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, may be an essential component of the primary miRNAs processing complex, along with Drosha, promoting the processing of primary microRNA to precursor microRNA. It is ubiquitous expressed in human and mouse tissues, and is deleted in DiGeorge syndrome[22323604]. The calculated molecular weight of DGCR8 is 82-86 kDa, but the post-modified DGCR8 is about 120 kDa.
Protocols
Product Specific Protocols
WB protocol for DGCR8 N-terminal antibody 25835-1-AP
WB result of DGCR8 antibody (25835-1-AP, 1:2000) with si-control and si-DGCR8 transfected A431 cells.
WB analysis of HeLa using 25835-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 25835-1-AP (DGCR8 N-terminal antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 25835-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 25835-1-AP (DGCR8 N-terminal antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 25835-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 25835-1-AP (DGCR8 N-terminal antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 25835-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 25835-1-AP (DGCR8 N-terminal antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 25835-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 25835-1-AP (DGCR8 N-terminal antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human ovary cancer using 25835-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25835-1-AP (DGCR8 N-terminal antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of A431 using 25835-1-AP
IP result of anti-DGCR8 N-terminal (IP:25835-1-AP, 4ug; Detection:25835-1-AP 1:600) with A431 cells lysate 2800ug.
IF/ICC Figures
IF Staining of HeLa using 25835-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 25835-1-AP (DGCR8 N-terminal antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
DGCR8 N-terminal Polyclonal antibody
Catalog Number
25835-1-AP
Citations
2
Dilutions
WB : 1:500-1:1000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:20-1:200
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, bovine
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
