DGCR8 N-terminal Polyclonal antibody

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• KD/KO Validated

DGCR8 N-terminal Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 25835-1-AP

Synonyms

C22orf12, DGCR8, DGCRK6, Gy1

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Validation Data Gallery

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  WB analysis of A431 cells using 25835-1-AP

  WB result of DGCR8 antibody (25835-1-AP, 1:2000) with si-control and si-DGCR8 transfected A431 cells.

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  WB analysis of HeLa using 25835-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25835-1-AP (DGCR8 N-terminal antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 25835-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25835-1-AP (DGCR8 N-terminal antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 25835-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25835-1-AP (DGCR8 N-terminal antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 25835-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 25835-1-AP (DGCR8 N-terminal antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 25835-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25835-1-AP (DGCR8 N-terminal antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 25835-1-AP

  IP Result of anti-DGCR8 N-terminal (IP:25835-1-AP, 4ug; Detection:25835-1-AP 1:600) with A431 cells lysate 2800ug.

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  IF Staining of HeLa using 25835-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 25835-1-AP (DGCR8 N-terminal antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, A431 cells
• Positive IP detected in: A431 cells
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• IF: See 2 publications below

Product Information

The immunogen of 25835-1-AP is DGCR8 N-terminal Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: mouse, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DGCR8 N-terminal fusion protein Ag22988
• Full Name: DiGeorge syndrome critical region gene 8
• Calculated molecular weight: 773 aa, 86 kDa
• Observed molecular weight: 88-100 kDa
• GenBank accession number: BC078147
• Gene symbol: DGCR8
• Gene ID (NCBI): 54487
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DGCR8 is a RNA-binding protein that assists the Rnase III enzyme Drosha in the processing of microRNAs (miRNAs), which regulate the expression of a large number of protein-coding genes[PMID: 22580560]. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, may be an essential component of the primary miRNAs processing complex, along with Drosha, promoting the processing of primary microRNA to precursor microRNA. It is ubiquitous expressed in human and mouse tissues, and is deleted in DiGeorge syndrome[22323604]. The calculated molecular weight of DGCR8 is 82-86 kDa, but the post-modified DGCR8 is about 120 kDa.

Publications

Species	Application	Title
mouse	IF	Nucleic Acids Res; DAZL regulates proliferation of human primordial germ cells by direct binding to precursor miRNAs and enhances DICER processing activity; Authors - An Yan
bovine	IF	Proc Natl Acad Sci U S A; Embryonic microRNAs are essential for bovine preimplantation embryo development; Authors - Erika E Paulson