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  • KD/KO Validated

DGCR8 C-terminal Polyclonal antibody

DGCR8 C-terminal Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, RIP, IP, IHC, IF, FC, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 10996-1-AP

Synonyms

C22orf12, DGCR8, DGCR8 C-terminal, DGCRK6, Gy1



Tested Applications

Positive WB detected inHEK-293 cells, A431 cells, Jurkat cells, HeLa cells, mouse testis tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells
Positive FC detected inHeLa cells, SH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10996-1-AP targets DGCR8 C-terminal in WB, RIP, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DGCR8 C-terminal fusion protein Ag1429
Full Name DiGeorge syndrome critical region gene 8
Calculated molecular weight 773 aa, 86 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC009323
Gene symbol DGCR8
Gene ID (NCBI) 54487
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DGCR8 is a RNA-binding protein that assists the Rnase III enzyme Drosha in the processing of microRNAs (miRNAs), which regulate the expression of a large number of protein-coding genes[PMID: 22580560]. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, may be an essential component of the primary miRNAs processing complex, along with Drosha, promoting the processing of primary microRNA to precursor microRNA. It is ubiquitous expressed in human and mouse tissues, and is deleted in DiGeorge syndrome[22323604]. The calculated molecular weight of DGCR8 is 82-86 kDa, but the post-modified DGCR8 is about 120 kDa (PMID: 18469815).

Protocols

Product Specific Protocols
WB protocol for DGCR8 C-terminal antibody 10996-1-APDownload protocol
IHC protocol for DGCR8 C-terminal antibody 10996-1-APDownload protocol
IF protocol for DGCR8 C-terminal antibody 10996-1-APDownload protocol
IP protocol for DGCR8 C-terminal antibody 10996-1-APDownload protocol
FC protocol for DGCR8 C-terminal antibody 10996-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,humanWB,IF

Nature

Global miRNA dosage control of embryonic germ layer specification.

Authors - Yingzi Cui
  • KO Validated
humanWB

Nature

Modulation of microRNA processing by p53.

Authors - Suzuki Hiroshi I HI
humanWB

Cell

Lin28A and Lin28B inhibit let-7 microRNA biogenesis by distinct mechanisms.

Authors - Piskounova Elena E
humanWB

Cell

Hippo signaling regulates microprocessor and links cell-density-dependent miRNA biogenesis to cancer.

Authors - Masaki Mori
mouseChIP

Cell

Super-Enhancer-Mediated RNA Processing Revealed by Integrative MicroRNA Network Analysis.

Authors - Hiroshi I Suzuki
mouseWB

Cell Stem Cell

An Intermediate Pluripotent State Controlled by MicroRNAs Is Required for the Naive-to-Primed Stem Cell Transition.

Authors - Peng Du