DFNA5 Recombinant antibody, PBS Only (Detector)

DFNA5 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240394E11

Cat no : 83454-4-PBS

Synonyms

Gasdermin-E, Gasdermin E, DFNA5/GSDME, GSDME, DFNA 5

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP00455-2

  Sandwich ELISA standard curve of MP00455-2, Human DFNA5 Recombinant Matched Antibody Pair - PBS only. 83454-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3746. 83454-4-PBS was HRP conjugated as the detection antibody. Range: 1.88-120 ng/mL

• 

  Sample test of MP00455-2

  HCT116 cell lysate was measured. The human DFNA5 concentration of detected samples was determined to be 5.90 µg/mL (based on a 4.3 mg/mL extract load).

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  Cytometric bead array standard curve of MP00455-1

  Cytometric bead array standard curve of MP00455-1, DFNA5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83454-5-PBS. Detection antibody: 83454-4-PBS. Standard: Ag3746. Range: 0.78-100 ng/mL.

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  Cytometric bead array standard curve of MP00455-2

  Cytometric bead array standard curve of MP00455-2, DFNA5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83454-1-PBS. Detection antibody: 83454-4-PBS. Standard: Ag3746. Range: 0.78-100 ng/mL.

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Product Information

83454-4-PBS targets DFNA5 as part of a matched antibody pair:

MP00455-1: 83454-5-PBS capture and 83454-4-PBS detection (validated in Cytometric bead array)

MP00455-2: 83454-1-PBS capture and 83454-4-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: DFNA5 fusion protein Ag3746
• Full Name: deafness, autosomal dominant 5
• Calculated Molecular Weight: 496 aa, 55 kDa
• GenBank Accession Number: BC019689
• Gene Symbol: DFNA5
• Gene ID (NCBI): 1687
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.