DFFA,DFF45 Polyclonal antibody

• Featured Product
• KD/KO Validated

DFFA,DFF45 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 10191-2-AP

Synonyms

DFF45, DFFA, DFF45/DFFA, DFF-45, DFF1

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Validation Data Gallery

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  WB analysis using 10191-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10191-2-AP (DFF45/DFFA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 10191-2-AP

  IP result of anti-DFF45/DFFA (IP:10191-2-AP, 3ug; Detection:10191-2-AP 1:1000) with HEK-293 cells lysate 2500ug.

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  IHC staining of human prostate cancer using 10191-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10191-2-AP (DFF45/DFFA antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HEK-293 using 10191-2-AP

  Immunofluorescent analysis of HEK-293 cells using 10191-2-AP (DFF45/DFFA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, HEK-293 cells, SKOV-3 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

10191-2-AP targets DFFA,DFF45 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DFFA,DFF45 fusion protein Ag0244
• Full Name: DNA fragmentation factor, 45kDa, alpha polypeptide
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 37 kDa, 45 kDa
• GenBank Accession Number: BC000037
• Gene Symbol: DFFA
• Gene ID (NCBI): 1676
• RRID: AB_2292937
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Apoptosis is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF), heterodimer of 40-kDa (DFFB) and 45-kDa (DFFA) subunits, is one of the major endonucleases responsible for internucleosomal DNA cleavage during apoptosis. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF activated after cleavage of DFFA by caspase-3 followed by its dissociation from DFFB. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. DFFA appears to be a 45-kDa protein despite the predicated MW of 37 kDa.

Publications

Species	Application	Title
human	WB	Mol Cancer; MiR-145, a new regulator of the DNA fragmentation factor-45 (DFF45)-mediated apoptotic network.; Authors - Zhang Jianjun J ; KD Validated
human	WB	Mol Med Rep; Polyphyllin VII induces apoptotic cell death via inhibition of the PI3K/Akt and NF‑κB pathways in A549 human lung cancer cells.; Authors - Hao He