DEPDC6/Deptor Polyclonal antibody

DEPDC6/Deptor Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 20985-1-AP

Synonyms

DEP domain containing 6, DEP.6, DEPDC6, DEPDC6/Deptor, Deptor

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Validation Data Gallery

• 

  WB analysis of HeLa using 20985-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20985-1-AP (DEPDC6/Deptor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 20985-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 20985-1-AP (DEPDC6/Deptor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 20985-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 20985-1-AP (DEPDC6/Deptor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A2780 using 20985-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 20985-1-AP (DEPDC6/Deptor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 20985-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20985-1-AP (DEPDC6/Deptor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 20985-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20985-1-AP (DEPDC6/Deptor antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 20985-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20985-1-AP (DEPDC6/Deptor antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver cancer using 20985-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 20985-1-AP (DEPDC6/Deptor antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 20985-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 20985-1-AP (DEPDC6/Deptor antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human thyroid cancer using 20985-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer using 20985-1-AP (DEPDC6/Deptor antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human thyroid cancer using 20985-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer using 20985-1-AP (DEPDC6/Deptor antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 20985-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 20985-1-AP (DEPDC6/Deptor antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 20985-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 20985-1-AP (DEPDC6/Deptor antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 20985-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DEPDC6/Deptor antibody (20985-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, A2780 cells, COLO 320 cells, HEK-293 cells, MCF-7 cells
• Positive IHC detected in: human liver cancer tissue, human thyroid cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

20985-1-AP targets DEPDC6/Deptor in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: DEP domain containing 6
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: NM_022783
• Gene Symbol: DEPDC6
• Gene ID (NCBI): 64798
• RRID: AB_11182391
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DEPDC6, also named as DEPTOR, was identified as a novel mTOR-interacting protein whose over-expression in certain cell types represents a new mechanism for activating PI3K/Akt signaling and promoting cell survival. It negative regulator of the mTORC1 and mTORC2 signaling pathways. DEPDC6 inhibits the kinase activity of both complexes. The antibody is specific to DEPDC6.

Publications

Species	Application	Title
human	WB,IHC	Sci Transl Med; The PTH/PTHrP-SIK3 pathway affects skeletogenesis through altered mTOR signaling.; Authors - Fabiana Csukasi
human	WB	Autophagy; TNFAIP3-DEPTOR complex regulates inflammasome secretion through autophagy in ankylosing spondylitis monocytes.; Authors - Yue Zhai
human	WB	Cell Death Differ; NUMB facilitates autophagy initiation through targeting SCFβ-TrCP2 complex.; Authors - Hao Li
mouse	WB	Oncotarget; Inhibition of neddylation regulates dendritic cell functions via Deptor accumulation driven mTOR inactivation.; Authors - Mengmeng Cheng
mouse	IF	Stem Cell Res Ther; DEPTOR regulates osteogenic differentiation via inhibiting MEG3-mediated activation of BMP4 signaling and is involved in osteoporosis.; Authors - Si Chen
mouse	WB	Gene; p65 down-regulates DEPTOR expression in response to LPS stimulation in hepatocytes.; Authors - Xiaoling Yu