DEK Polyclonal antibody

• Featured Product
• KD/KO Validated

DEK Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Conjugate

Unconjugated

Publications

24

Reviews (1)

Cat no : 16448-1-AP

Synonyms

D6S231E

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Validation Data Gallery

• 

  WB analysis using 16448-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16448-1-AP (DEK antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16448-1-AP

  WB result of DEK antibody (16448-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DEK transfected HEK-293 cells.

• 

  IP experiment of HeLa using 16448-1-AP

  IP result of anti-DEK (IP:16448-1-AP, 4ug; Detection:16448-1-AP 1:500) with HeLa cells lysate 1200ug.

• 

  IHC staining of mouse brain using 16448-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16448-1-AP (DEK antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 16448-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16448-1-AP (DEK antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 16448-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16448-1-AP (DEK antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 16448-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16448-1-AP (DEK antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 16448-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DEK antibody (16448-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, K-562 cells, T-47D cells, C6 cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 15 publications below
• IHC: See 6 publications below
• IF: See 5 publications below
• ChIP: See 1 publications below

Product Information

16448-1-AP targets DEK in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DEK fusion protein Ag8225
• Full Name: DEK oncogene
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 43-50 kDa
• GenBank Accession Number: BC035259
• Gene Symbol: DEK
• Gene ID (NCBI): 7913
• RRID: AB_2092097
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Dek oncogene(DEK) is first recognized in a fusion with CAN nucleoporin protein, and it's a also autoantigen in patients with pquciarticular onset juvenile rheumatoid arthritis. As a site-specific binding protein, DEK may involve in transcriptional regulation and signal transduction. Analysis by agarose gel celctrophoresis, DEK showed to reduce the efficiency with which chromation is replicated. It's one of the first components of the splicing complex, which remains associate with spliced exons dependent on prior splicing of pre-mRNA.

Publications

Species	Application	Title
mouse	IF	Nature; Maintenance of muscle stem-cell quiescence by microRNA-489.; Authors - Cheung Tom H TH
mouse	IF	Dev Cell; Hira-mediated H3.3 incorporation is required for DNA replication and ribosomal RNA transcription in the mouse zygote.; Authors - Chih-Jen Lin
mouse	WB	J Exp Med; Nuclear DEK preserves hematopoietic stem cells potential via NCoR1/HDAC3-Akt1/2-mTOR axis.; Authors - Zhe Chen
human	WB	Dev Cell; Dek Modulates Global Intron Retention during Muscle Stem Cells Quiescence Exit.; Authors - Lu Yue
mouse	WB	Oncogene; Overexpression of miR-489 derails mammary hierarchy structure and inhibits HER2/neu-induced tumorigenesis.; Authors - Y Patel
human,mouse	WB,IF,ChIP	Oncogene; Exosomal DEK removes chemoradiotherapy resistance by triggering quiescence exit of breast cancer stem cells.; Authors - Yao-Shun Yang ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Emma (Verified Customer) (11-29-2021)

Nice antibody which worked well by IF on PC3 cells fixed in 4% PFA.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:25
• Cell Tissue Type: PC3 cells

DEK Antibody Immunofluorescence validation (1:25 dilution) in PC3 cells (Cat no:16448-1-AP)

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