DEFB1 Polyclonal antibody

DEFB1 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 14738-1-AP

Synonyms

BD 1, BD1, Beta defensin 1, DEFB 1, DEFB1, DEFB101, defensin, beta 1, hBD 1, HBD1

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Validation Data Gallery

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  IHC staining of human colon cancer using 14738-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14738-1-AP (DEFB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 14738-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14738-1-AP (DEFB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 2 publications below
• ELISA: See 1 publications below

Product Information

14738-1-AP targets DEFB1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DEFB1 fusion protein Ag6406
• Full Name: defensin, beta 1
• Calculated Molecular Weight: 7 kDa
• GenBank Accession Number: BC047677
• Gene Symbol: DEFB1
• Gene ID (NCBI): 1672
• RRID: AB_2091826
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Sci Rep; β-defensin 1 expression in HCV infected liver/liver cancer: an important role in protecting HCV progression and liver cancer development.; Authors - Yue-Ming Ling
human	WB	Indian J Gastroenterol; Antibacterial spectrum of human omentum and differential expression of beta defensins.; Authors - Meenu Srivastava
human	WB	Cureus; Expression of Antimicrobial Peptides and Cytokines in Human Omentum Following Abdominal Surgery; Authors - Meenu Srivastava
mouse	ELISA,IHC,WB	Biomed Pharmacother; Shen Qi Wan ameliorates nephritis in chronic kidney disease via AQP1 and DEFB1 regulation; Authors - Yiming Liu

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