DEFA1 Polyclonal antibody

DEFA1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 18057-1-AP

Synonyms

HP 1-56, HNP-1, defensin, alpha 1, DEFA2, DEFA1B

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Validation Data Gallery

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  WB analysis of HepG2 using 18057-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18057-1-AP (DEFA1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human small intestine using 18057-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 18057-1-AP (DEFA1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human small intestine using 18057-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 18057-1-AP (DEFA1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 18057-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18057-1-AP (DEFA1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 18057-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18057-1-AP (DEFA1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 18057-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 18057-1-AP (DEFA1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells
• Positive IHC detected in: human small intestine tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Product Information

18057-1-AP targets DEFA1 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DEFA1 fusion protein Ag12592
• Full Name: defensin, alpha 1
• Calculated Molecular Weight: 94 aa, 10 kDa
• Observed Molecular Weight: 6 kDa
• GenBank Accession Number: BC093791
• Gene Symbol: DEFA1
• Gene ID (NCBI): 1667
• RRID: AB_2292842
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,CoIP	Life Sci; DEFA1B inhibits ZIKV replication and retards cell cycle progression through interaction with ORC1.; Authors - Shuang Li
human	WB	Hum Cell; Histone deacetylase 6-mediated downregulation of TMEM100 expedites the development and progression of non-small cell lung cancer.; Authors - Yanyun Wang
human	IHC	Syst Biol Reprod Med; Identification of differentially expressed proteins associated with recurrence in ovarian endometriotic cysts.; Authors - Genhai Zhu
human	WB	Mediators Inflamm; Potential Association of Gut Microbial Metabolism and Circulating mRNA Based on Multiomics Sequencing Analysis in Fetal Growth Restriction; Authors - Hui Tang