DEC1 Polyclonal antibody

DEC1 Polyclonal Antibody for IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 25021-1-AP

Synonyms

CTS9, DEC1, DELEC1, deleted in esophageal cancer 1

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Validation Data Gallery

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  IHC staining of human prostate cancer using 25021-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25021-1-AP (DEC1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 25021-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25021-1-AP (DEC1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 25021-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25021-1-AP (DEC1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 25021-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25021-1-AP (DEC1 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human prostate cancer tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 25021-1-AP is DEC1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DEC1 fusion protein Ag20192
• Full Name: deleted in esophageal cancer 1
• Calculated Molecular Weight: 70 aa, 8 kDa
• GenBank Accession Number: BC153139
• Gene Symbol: DEC1
• Gene ID (NCBI): 50514
• RRID: AB_2879851
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DEC1 (Deleted in esophageal cancer 1), also named as CTS9 (Candidate tumor suppressor CTS9),is a tumor suppressor protein. It is expressed in many tissues, with highest expression in prostate and testis. This gene is different to another DEC1 protein (Differentially expressed in chondrocytes protein 1, BHLHE40).

Publications

Species	Application	Title
rat	WB	FASEB J; Persistent myopathy despite release of partial obstruction: in vivo reversal of dysfunction and transcriptional responses using rapamycin.; Authors - Annette Schröder