DDX60 Recombinant antibody, PBS Only

DDX60 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230293C10

Cat no : 82969-1-PBS

Synonyms

Probable ATP-dependent RNA helicase DDX60, EC:3.6.4.13, DEAD box protein 60, 230293C10

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Validation Data Gallery

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  WB analysis using 82969-1-RR (same clone as 82969-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82969-1-RR (DDX60 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82969-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 82969-1-RR (same clone as 82969-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 82969-1-RR (DDX60 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82969-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 82969-1-RR (same clone as 82969-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using DDX60 antibody (82969-1-RR, Clone: 230293C10 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 82969-1-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 82969-1-RR (same clone as 82969-1-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.2 ug DDX60 Recombinant antibody (82969-1-RR, Clone:230293C10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82969-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82969-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82969-1-RR against Human DDX60 were performed. The affinity constant is 0.145 nM.

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Product Information

82969-1-PBS targets DDX60 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: DDX60 fusion protein Ag34406
• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 60
• Calculated Molecular Weight: 1712 aa, 198 kDa
• Observed Molecular Weight: 170-200 kDa
• GenBank Accession Number: BC038115
• Gene Symbol: DDX60
• Gene ID (NCBI): 55601
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

DDX60, also called FLJ20035, is an IFN-inducible gene that has been identified via a microarray analysis of genes induced by viral infection in human dendritic cells (DCs). DDX60 expression correlated strongly with immune checkpoint and immune system-related metagene clusters, and DDX60 promoted cell proliferation, migration, and invasion and was related to poor prognosis and immune resistance(PMID: 37274827). Involved in RIG-I-dependent and independent innate immune responses, DDX60 has been proven to be associated with the development of tumors.