DDX60 Polyclonal antibody

DDX60 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 30982-1-AP

Synonyms

Probable ATP-dependent RNA helicase DDX60, EC:3.6.4.13, DEAD box protein 60

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Validation Data Gallery

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  WB analysis using 30982-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30982-1-AP (DDX60 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 30982-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 30982-1-AP (DDX60 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 30982-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 30982-1-AP (DDX60 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U-251 using 30982-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using DDX60 antibody (30982-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: A549 cells, Jurkat cells, U-251 cells, U-87 MG cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

30982-1-AP targets DDX60 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DDX60 fusion protein Ag34406
• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 60
• Calculated Molecular Weight: 1712 aa, 198 kDa
• Observed Molecular Weight: 170-200 kDa
• GenBank Accession Number: BC038115
• Gene Symbol: DDX60
• Gene ID (NCBI): 55601
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DDX60, also called FLJ20035, is an IFN-inducible gene that has been identified via a microarray analysis of genes induced by viral infection in human dendritic cells (DCs). DDX60 expression correlated strongly with immune checkpoint and immune system-related metagene clusters, and DDX60 promoted cell proliferation, migration, and invasion and was related to poor prognosis and immune resistance(PMID: 37274827). Involved in RIG-I-dependent and independent innate immune responses, DDX60 has been proven to be associated with the development of tumors.

Publications

Species	Application	Title
human	WB	J Inflamm Res; Machine Learning Screening and Validation of PANoptosis-Related Gene Signatures in Sepsis; Authors - Jingjing Xu
human	WB	Front Cell Dev Biol; Genome-wide analysis reveals the MORC3-mediated repression of PD-L1 expression in head and neck cancer; Authors - Wenxuan Fu