DDX52 Polyclonal antibody

DDX52 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16660-1-AP

Synonyms

Probable ATP-dependent RNA helicase DDX52, HUSSY-19, HUSSY19, EC:3.6.4.13, DEAD box protein 52

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Validation Data Gallery

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  WB analysis using 16660-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16660-1-AP (DDX52 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 16660-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16660-1-AP (DDX52 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 16660-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 16660-1-AP (DDX52 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, Jurkat cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16660-1-AP targets DDX52 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DDX52 fusion protein Ag10118
• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 52
• Calculated Molecular Weight: 599 aa, 67 kDa
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: BC041785
• Gene Symbol: DDX52
• Gene ID (NCBI): 11056
• RRID: AB_1851052
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.