DDX5 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

DDX5 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10804-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of NIH/3T3 using 10804-1-AP (same clone as 10804-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10804-1-AP (DDX5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10804-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 10804-1-AP (same clone as 10804-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10804-1-AP (DDX5, antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10804-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 10804-1-AP (same clone as 10804-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 10804-1-AP (DDX5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10804-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 10804-1-AP (same clone as 10804-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10804-1-AP (DDX5 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10804-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 10804-1-AP (same clone as 10804-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10804-1-AP (DDX5 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10804-1-PBS in a different storage buffer formulation.

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Product Information

10804-1-PBS targets DDX5 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DDX5 fusion protein Ag1237
• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 5
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 65-68 kDa
• GenBank Accession Number: BC016027
• Gene Symbol: DDX5
• Gene ID (NCBI): 1655
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

DDX5 or p68, a member of DEAD/H BOX, which has a characteristic DEAD box, is a proliferation-associated nuclear antigen. DDX5 involves in the alternative regulation of pre-mRNA splicing, act as a RNA helicase to increase tau exon10 inclusion in a RBM4-dependent manner. It's also a transcription coactivator for extrogen receptor ESR1 and androgen receptor AR. Once synergized with DDX17 and SRA1 RNA, DDX5 activated MYOD1 transcription activity and involved in skeletal muscle differentiation. For the reason that DDX5 can be phosphorylated and dephosphorylated at several sites, at some cases the molecular weight of DDX5 is less than 68kD, approximately 65kd