DDX47 Polyclonal antibody
DDX47 Polyclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 17795-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, HepG2 cells, Jurkat cells, SH-SY5Y cells |
Positive IF/ICC detected in | U2OS cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:8000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
17795-1-AP targets DDX47 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | DDX47 fusion protein Ag11953 |
Full Name | DEAD (Asp-Glu-Ala-Asp) box polypeptide 47 |
Calculated Molecular Weight | 455 aa, 51 kDa |
Observed Molecular Weight | 48 kDa |
GenBank Accession Number | BC068009 |
Gene Symbol | DDX47 |
Gene ID (NCBI) | 51202 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DEAD-box helicase 47 (DDX47) is a member of the DEAD-box protein family. It contains two conserved domains: DEXDc (DEAD-like helicases superfamily) and HELICc (helicase superfamily c-terminal domain) (PMID: 16337753). DDX47 can shuttle between the nucleus and the cytoplasm. In different cancers, evidence has shown that DDX47 is a poor prognostic predictor for renal cell carcinoma, as well as a diagnostic biomarker for breast cancer and chronic myeloid leukemia (PMID: 35490652).
Protocols
Product Specific Protocols | |
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WB protocol for DDX47 antibody 17795-1-AP | Download protocol |
IF protocol for DDX47 antibody 17795-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |