DDX47 Polyclonal antibody

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  WB analysis using 17795-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17795-1-AP (DDX47 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 17795-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using DDX47 antibody (17795-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

DDX47 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 17795-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

DDX47, DEAD box protein 47, E4 DBP, HQ0256, MSTP162

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, Jurkat cells, SH-SY5Y cells
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17795-1-AP targets DDX47 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DDX47 fusion protein Ag11953
• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 47
• Calculated Molecular Weight: 455 aa, 51 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC068009
• Gene Symbol: DDX47
• Gene ID (NCBI): 51202
• RRID: AB_3085540
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H0S4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DEAD-box helicase 47 (DDX47) is a member of the DEAD-box protein family. It contains two conserved domains: DEXDc (DEAD-like helicases superfamily) and HELICc (helicase superfamily c-terminal domain) (PMID: 16337753). DDX47 can shuttle between the nucleus and the cytoplasm. In different cancers, evidence has shown that DDX47 is a poor prognostic predictor for renal cell carcinoma, as well as a diagnostic biomarker for breast cancer and chronic myeloid leukemia (PMID: 35490652).