DDX41 Polyclonal antibody

DDX41 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 27500-1-AP

Synonyms

Probable ATP-dependent RNA helicase DDX41, EC:3.6.4.13, DEAD box protein 41, ABS

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Validation Data Gallery

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  WB analysis of MCF-7 using 27500-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 27500-1-AP (DDX41 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 27500-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 27500-1-AP (DDX41 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 27500-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 27500-1-AP (DDX41 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 27500-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27500-1-AP (DDX41 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: MCF-7 cells, RAW 264.7 cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:750-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

27500-1-AP targets DDX41 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DDX41 fusion protein Ag26593
• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 41
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC015476
• Gene Symbol: DDX41
• Gene ID (NCBI): 51428
• RRID: AB_2880891
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DDX41, also named as DEAD box protein abstrakt homolog, is a 622 amino acid protein, which is a probable ATP-dependent RNA helicase. DDX41 is required during post-transcriptional gene expression.and may be involved in pre-mRNA splicing.

Publications

Species	Application	Title
mouse	WB	J Transl Med; MicroRNA-218-5p-Ddx41 axis restrains microglia-mediated neuroinflammation through downregulating type I interferon response in a mouse model of Parkinson's disease; Authors - Danlei Wang