DDX4,VASA Monoclonal antibody

DDX4,VASA Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

3

CloneNo.

1F12C3

Cat no : 67147-2-Ig

Synonyms

DDX4, DDX4,VASA, DEAD box protein 4, VASA, Vasa homolog

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Validation Data Gallery

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  WB analysis of SKOV-3 using 67147-2-Ig

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 67147-2-Ig (DDX4,VASA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 67147-2-Ig

  rat testis tissue were subjected to SDS PAGE followed by western blot with 67147-2-Ig (DDX4,VASA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of JAR using 67147-2-Ig

  JAR cells were subjected to SDS PAGE followed by western blot with 67147-2-Ig (DDX4,VASA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NCCIT using 67147-2-Ig

  NCCIT cell were subjected to SDS PAGE followed by western blot with 67147-2-Ig (DDX4,VASA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 67147-2-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67147-2-Ig (DDX4,VASA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse testis using 67147-2-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using DDX4,VASA antibody (67147-2-Ig, Clone: 1F12C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: SKOV-3 cells, JAR cells, rat testis tissue, NCCIT cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 2 publications below

Product Information

67147-2-Ig targets DDX4,VASA in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: DDX4,VASA fusion protein Ag11630
• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
• Calculated Molecular Weight: 690aa,76 kDa; 724aa,79 kDa
• Observed Molecular Weight: 79 kDa
• GenBank Accession Number: BC047455
• Gene Symbol: DDX4
• Gene ID (NCBI): 54514
• RRID: AB_2882446
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DEAD box proteins are characterized by nine conserved sequence motifs located on two functional domains. Domain I contains six of these motifs, including the Q motif and the Walker A motif, motifs Ia and Ib, the Walker B motif, and motif III, which may act to link ATPase and helicase activities of the protein [PMID:21653890]. DDX4, a member of the DEAD box family of ATP-dependent RNA helicases, plays a central role in several aspects of germ cell development. Its function is not only required during gametogenesis in the adult but is also essential for the specification of the germ cell lineage during embryogenesis [PMID:20016130].

Publications

Species	Application	Title
mouse	IF	Reproduction; NR2C2 of macrophages promotes inflammation via NF-κB in LPS-induced orchitis in mice; Authors - Ziqian Min
rat,mouse,human		iScience; Chromodomain helicase DNA-binding domain 2 maintains spermatogonial self-renewal by promoting chromatin accessibility and mRNA stability; Authors - Ziqian Min
rat	IF	Reprod Sci; Mechanism Research of DHEA Treatment Improving Diminished Ovarian Reserve by Attenuating the AMPK-SIRT1 Signaling and Mitophagy; Authors - Qianwen Ma