DDX4,VASA Polyclonal antibody

DDX4,VASA Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

11

Reviews (1)

Cat no : 51042-1-AP

Synonyms

DDX4, DDX4,VASA, DEAD box protein 4, VASA, Vasa homolog

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Validation Data Gallery

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  WB analysis using 51042-1-AP

  Mouse testis tissue were subjected to SDS PAGE followed by western blot with 51042-1-AP (DDX4,VASA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 51042-1-AP

  IP result of anti-DDX4,VASA (IP:51042-1-AP, 4ug; Detection:51042-1-AP 1:500) with mouse testis tissue lysate 2040 ug.

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  IHC staining of mouse testis using 51042-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 51042-1-AP (DDX4,VASA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 51042-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 51042-1-AP (DDX4,VASA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 51042-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 51042-1-AP (DDX4,VASA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 51042-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 51042-1-AP (DDX4,VASA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 51042-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 51042-1-AP (DDX4,VASA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse testis using 51042-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using DDX4,VASA antibody (51042-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse testis using 51042-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using DDX4,VASA antibody (51042-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: mouse testis tissue, mouse ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 4 publications below
• IF: See 5 publications below

Product Information

51042-1-AP targets DDX4,VASA in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DDX4,VASA fusion protein Ag0447
• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
• Calculated Molecular Weight: 690aa,76 kDa; 724aa,79 kDa
• Observed Molecular Weight: 79 kDa
• GenBank Accession Number: BC047455
• Gene Symbol: DDX4
• Gene ID (NCBI): 54514
• RRID: AB_2092998
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DEAD box proteins are characterized by nine conserved sequence motifs located on two functional domains. Domain I contains six of these motifs, including the Q motif and the Walker A motif, motifs Ia and Ib, the Walker B motif, and motif III, which may act to link ATPase and helicase activities of the protein [PMID:21653890]. DDX4, a member of the DEAD box family of ATP-dependent RNA helicases, plays a central role in several aspects of germ cell development. Its function is not only required during gametogenesis in the adult but is also essential for the specification of the germ cell lineage during embryogenesis [PMID:20016130].

Publications

Species	Application	Title
mouse,rat	WB,IF	Environ Pollut; Wnt10a downregulation contributes to MEHP-induced disruption of self-renewal and differentiation balance and proliferation inhibition in GC-1 cells: Insights from multiple transcriptomic profiling; Authors - Yifan Hong
human	WB	Oncotarget; Generation and characteristics of human Sertoli cell line immortalized by overexpression of human telomerase.; Authors - Liping Wen
mouse	IHC,IF	Sci China Life Sci; Proteome landscape and spatial map of mouse primordial germ cells.; Authors - Pan Wang
mouse	WB,IHC	J Pharmacol Sci; Berberine protects cyclophosphamide and busulfan-induced premature ovarian insufficiency in mouse model; Authors - Ying Peng
mouse	IF	STAR Protoc; Optimized protocol for isolation of germ cells from mouse testis by centrifugal elutriation.; Authors - Yuzhu Cao
mouse	IHC	Int J Mol Sci; Tracking Immature Testicular Tissue after Vitrification In Vitro and In Vivo for Pre-Pubertal Fertility Preservation: A Translational Transgenic Mouse Model; Authors - Buo-Jia Lu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hetty (Verified Customer) (04-10-2018)

very good antibody.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse testis lysate