DDX4,VASA Polyclonal antibody

DDX4,VASA Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17545-1-AP

Synonyms

DDX4, DDX4,VASA, DEAD box protein 4, VASA, Vasa homolog

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Validation Data Gallery

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  WB analysis using 17545-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17545-1-AP (DDX4,VASA antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 17545-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 17545-1-AP (DDX4,VASA antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 17545-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17545-1-AP (DDX4,VASA antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 17545-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17545-1-AP (DDX4,VASA antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse testis tissue, rat testis tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 17545-1-AP is DDX4,VASA Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DDX4,VASA fusion protein Ag11647
• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
• Calculated Molecular Weight: 690aa,76 kDa; 724aa,79 kDa
• Observed Molecular Weight: 79 kDa
• GenBank Accession Number: BC047455
• Gene Symbol: DDX4
• Gene ID (NCBI): 54514
• RRID: AB_2918053
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DEAD box proteins are characterized by nine conserved sequence motifs located on two functional domains. Domain I contains six of these motifs, including the Q motif and the Walker A motif, motifs Ia and Ib, the Walker B motif, and motif III, which may act to link ATPase and helicase activities of the protein [PMID:21653890]. DDX4, a member of the DEAD box family of ATP-dependent RNA helicases, plays a central role in several aspects of germ cell development. Its function is not only required during gametogenesis in the adult but is also essential for the specification of the germ cell lineage during embryogenesis [PMID:20016130].

Publications

Species	Application	Title
		Sci Rep; FACS-sorted putative oogonial stem cells from the ovary are neither DDX4-positive nor germ cells.; Authors - Larissa Zarate-Garcia