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  • KD/KO Validated

DDX3 Polyclonal antibody

DDX3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Cat no : 11115-1-AP

Synonyms

DDX3X, DEAD box protein 3, X-chromosomal, DDX14, DBX, CAP-Rf



Tested Applications

Positive WB detected inHeLa cells, NIH/3T3 cells, HepG2 cells, mouse liver tissue, HT-1080 cells, mouse brain tissue, PC-12 cells, K-562 cells, C6 cells, rat brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman malignant melanoma tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11115-1-AP targets DDX3 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DDX3 fusion protein Ag1614
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked
Calculated Molecular Weight 73 kDa
Observed Molecular Weight73 kDa
GenBank Accession NumberBC011819
Gene Symbol DDX3
Gene ID (NCBI) 1654
RRIDAB_10896499
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DDX3X, also named as DBX, DDX3 and HLP2, belongs to the DEAD box helicase family and DDX3/DED1 subfamily. It is ATP-dependent RNA helicase. DDX3X acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. It is also involved in HIV-1 replication. DDX3X interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location. 73 kDa is the target band. 60-65 kDa is some other isoform. This antibody can bind both DDX3X and DDX3Y for the close sequences.

Protocols

Product Specific Protocols
WB protocol for DDX3 antibody 11115-1-APDownload protocol
IHC protocol for DDX3 antibody 11115-1-APDownload protocol
IF protocol for DDX3 antibody 11115-1-APDownload protocol
IP protocol for DDX3 antibody 11115-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Neuron

Pathogenic DDX3X Mutations Impair RNA Metabolism and Neurogenesis during Fetal Cortical Development.

Authors - Ashley L Lennox
humanWB

Neuron

CRISPR-Cas9 Screens Identify the RNA Helicase DDX3X as a Repressor of C9ORF72 (GGGGCC)n Repeat-Associated Non-AUG Translation.

Authors - Weiwei Cheng
  • KD Validated
humanWB

Cancer Lett

NR4A1 depletion inhibits colorectal cancer progression by promoting necroptosis via the RIG-I-like receptor pathway

Authors - Jinghan Zhu
humanWB

Pharmacol Res

HPV E7-drived ALKBH5 promotes cervical cancer progression by modulating m6A modification of PAK5

Authors - Fu-Chun Huo
  • KD Validated
mouseWB,IF,CoIP

Ecotoxicol Environ Saf

Aluminum exposure induces central nervous system impairment via activating NLRP3-medicated pyroptosis pathway

Authors - Wudi Hao
WB,IF

J Virol

DDX3X Is Hijacked by Snakehead Vesiculovirus Phosphoprotein To Facilitate Virus Replication via Stabilization of the Phosphoprotein

Authors - Congran Bei

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Shannon (Verified Customer) (07-19-2022)

Worked well, also binds to different isoforms/protein dimers

  • Applications: Western Blot, Immunoprecipitation
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Jurkat T-Cells
DDX3 Antibody Western Blot,Immunoprecipitation validation (1:2000 dilution) in Jurkat T-Cells (Cat no:11115-1-AP)