DDX24 Recombinant antibody, PBS Only

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  WB analysis using 84103-6-RR (same clone as 84103-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84103-6-RR (DDX24 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84103-6-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84103-6-RR (same clone as 84103-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DDX24 antibody (84103-6-RR, Clone: 241145G10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84103-6-PBS in a different storage buffer formulation.

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  FC experiment of U-2 OS using 84103-6-RR (same clone as 84103-6-PBS)

  1x10^6 U-2 OS were intracellularly stained with 0.25 ug DDX24 Recombinant antibody (84103-6-RR, Clone:241145G10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84103-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84103-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84103-6-RR against Human DDX24 were performed. The affinity constant is below 1 pM.

DDX24 {ptg:Brand} Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 84103-6-PBS

Clone No.241145G10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

EC:3.6.4.13, DDX 24, ATP-dependent RNA helicase DDX24, 241145G10

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Product Information

84103-6-PBS targets DDX24 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: DDX24 fusion protein Ag8407
• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 24
• Calculated Molecular Weight: 96 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC008847
• Gene Symbol: DDX24
• Gene ID (NCBI): 57062
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9GZR7
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

DDX24 is a family member of Asp-Glu-Ala-Asp (DEAD) box containing RNA helicases, and DEAD-box RNA helicases are characterized by a conserved DEAD motif. DDX24 was associated with cancer development, viral infection,and vascular malformation.