DDX24 Recombinant antibody

DDX24 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241145D11

Cat no : 84103-5-RR

Synonyms

EC:3.6.4.13, DDX 24, ATP-dependent RNA helicase DDX24, 241145D11

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Validation Data Gallery

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  WB analysis using 84103-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84103-5-RR (DDX24 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, COLO 320 cells, Caco-2 cells, HT-29 cells, THP-1 cells, Y79 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84103-5-RR is DDX24 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: DDX24 fusion protein Ag8407
• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 24
• Calculated Molecular Weight: 96 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC008847
• Gene Symbol: DDX24
• Gene ID (NCBI): 57062
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DDX24 is a family member of Asp-Glu-Ala-Asp (DEAD) box containing RNA helicases, and DEAD-box RNA helicases are characterized by a conserved DEAD motif. DDX24 was associated with cancer development, viral infection,and vascular malformation.