DDX19B Polyclonal antibody

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  WB analysis of K-562 using 18285-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 18285-1-AP (DDX19B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 18285-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18285-1-AP (DDX19B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 18285-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18285-1-AP (DDX19B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18285-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18285-1-AP (DDX19B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 18285-1-AP

  IP result of anti-DDX19B (IP:18285-1-AP, 4ug; Detection:18285-1-AP 1:500) with K-562 cells lysate 4000ug.

DDX19B Polyclonal Antibody for WB, IP, ELISA

Cat No. 18285-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

DBP5, DDX19, DDX19B, DEAD box protein 19B, DEAD box RNA helicase DEAD5, RNAh

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Tested Applications

• Positive WB detected in: K-562 cells, HEK-293 cells, HeLa cells, NIH/3T3 cells
• Positive IP detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

18285-1-AP targets DDX19B in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DDX19B fusion protein Ag13165
• Full Name: DEAD (Asp-Glu-Ala-As) box polypeptide 19B
• Calculated Molecular Weight: 479aa,54 kDa; 370aa,42 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC010008
• Gene Symbol: DDX19B
• Gene ID (NCBI): 11269
• RRID: AB_10597088
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UMR2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Acta Neuropathol Commun; Inefficient quality control of ribosome stalling during APP synthesis generates CAT-tailed species that precipitate hallmarks of Alzheimer's disease; Authors - Suman Rimal